A Simple and Rapid Method of Preparation of Rabbit Antiserum Against Alkyl Hydroperoxide Reductase (Ahpc) of Helicobacter pylori
نویسندگان
چکیده
منابع مشابه
preparative sds-page electroelution for rapid purification of alkyl hydroperoxide reductase from helicobacter pylori
background: alkyl hydroperoxide reductase (ahpc) of helicobacter pylori is considered as a diagnostic antigen. therefore, this antigen can be used to detect h. pylori infection by stool immunoassays such as elisa. the aim of this study was to simplify the ahpc protein purification procedures. methods: for whole cell protein extraction, the bacterial cells were ruptured by octly-β-d glucopyrano...
متن کاملPreparative SDS-PAGE Electroelution for Rapid Purification of Alkyl Hydroperoxide Reductase from Helicobacter pylori
BACKGROUND Alkyl hydroperoxide reductase (AhpC) of Helicobacter pylori is considered as a diagnostic antigen. Therefore, this antigen can be used to detect H. pylori infection by stool immunoassays such as ELISA. The aim of this study was to simplify the AhpC protein purification procedures. METHODS For whole cell protein extraction, the bacterial cells were ruptured by octly-β-D glucopyranos...
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15 صفحه اولRoles of alkyl hydroperoxide reductase subunit C (AhpC) in viable but nonculturable Vibrio parahaemolyticus.
Alkyl hydroperoxide reductase subunit C (AhpC) is the catalytic subunit responsible for the detoxification of reactive oxygen species that form in bacterial cells or are derived from the host; thus, AhpC facilitates the survival of pathogenic bacteria under environmental stresses or during infection. This study investigates the role of AhpC in the induction and maintenance of a viable but noncu...
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ژورنال
عنوان ژورنال: International Journal of Infectious Diseases
سال: 2008
ISSN: 1201-9712
DOI: 10.1016/j.ijid.2008.05.417